Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin

Figure 3

Prostasin increases CYP11B2 expression and aldosterone production independently of its proteolytic activity in H295R cells. (a) CYP11B2 expression was measured following 24-hour treatment with prostasin in the presence or absence of 100 μM camostat mesilate (CM). (b) Aldosterone production was determined following 48-hour treatment with prostasin in the presence or absence of 100 μM CM. (c) CYP11B2 expression was measured following 24-hour treatment with protease-dead mutant prostasin. (d) Aldosterone production was determined following 48-hour treatment with mutant prostasin. Values are means ± SD (𝑛=6). ^∗𝑃<.001 versus vehicle.

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