Hydrogen Peroxide Toxicity Induces Ras Signaling in Human Neuroblastoma SH-SY5Y Cultured Cells
Effect of an inhibitor of G-protein activation, GDP-beta-S, on H2O2-induced reduction in cell viability in SH-SY5Y cultured cells. SH-SY5Y cells were treated with 0.001, 0.01, 0.1, and 1.0 mM GDP-beta-S for 3 hours prior to incubation with 0.1 mM H2O2 for another 24 hours. Cell viability was assessed using MTT assay and presented as percentage of untreated (0 mM) control cells. The results are expressed as mean ± SEM of five independent experiments. The ANOVA was performed for statistical analysis (*P compared with control and #P compared with H2O2-treated cells).