Figure 2: Secreted gelsolin inhibits A 𝛽 -induced disruption on choroid plexus epithelial cell cytoskeleton. (a) Representative confocal images of choroid plexus confluent monolayer labeled with anti-ZO-1 antibody. Under control conditions, ZO-1 immunostaining is distributed along the plasma membrane. In contrast, after exposure to A 𝛽 1 - 4 2 for 48 hours, a disruption of the plasma membrane pattern of ZO-1 was observed, resulting in increased cytoplasmic localization. Note the ability of gelsolin treatment to prevent this A 𝛽 -induced alteration in ZO-1 pattern. ( 𝑛 = 3 ). (b) A 𝛽 1 - 4 2 treatment resulted in increased serine phosphorylation of ZO-1 and decreased ZO-1 expression in choroid plexus epithelial cells. Gelsolin coadministration markedly attenuated A 𝛽 1 - 4 2 alteration in ZO-1 ( 𝑛 = 3 ). (c) BIODIPY FL phallacidin staining of choroid plexus epithelial cells showed a disruption of the actin cytoskeleton after treatment with A 𝛽 1 - 4 2 for 48 hours, and reversion when gelsolin was simultaneously added. Magnification: × 40. Scale bars = 10  𝜇 m.