Figure 2: Secreted gelsolin inhibits -induced disruption on choroid plexus epithelial cell cytoskeleton. (a) Representative confocal images of choroid plexus confluent monolayer labeled with anti-ZO-1 antibody. Under control conditions, ZO-1 immunostaining is distributed along the plasma membrane. In contrast, after exposure to for 48 hours, a disruption of the plasma membrane pattern of ZO-1 was observed, resulting in increased cytoplasmic localization. Note the ability of gelsolin treatment to prevent this -induced alteration in ZO-1 pattern. (). (b) treatment resulted in increased serine phosphorylation of ZO-1 and decreased ZO-1 expression in choroid plexus epithelial cells. Gelsolin coadministration markedly attenuated alteration in ZO-1 (). (c) BIODIPY FL phallacidin staining of choroid plexus epithelial cells showed a disruption of the actin cytoskeleton after treatment with for 48 hours, and reversion when gelsolin was simultaneously added. Magnification: 40. Scale bars = 10 m.