Figure 3: Secreted gelsolin expression modulates NO production and cell death in choroid plexus epithelial cells. (a) Choroid plexus epithelial cells treated with for 48 hours exhibited a significantly enhanced NO production compared with untreated cells. Secreted gelsolin coadministration completely blocked this effect (); **. (b) Increased cell death was observed in choroid plexus cell cultures 48 hours after treatment, and gelsolin addition totally reversed this toxic effect (); *. (c and d) treatment reduced mitochondrial complex IV in-gel activity in choroid plexus epithelial cells, whereas secreted gelsolin administration increased complex IV activity and reversed this decrease in -induced activity. Blue native analysis of these culture samples showed altered protein expression in the mitochondrial complex IV. Representative blue native blots and quantitative histograms are shown ( per group); *.