BioMed Research International / 2010 / Article / Fig 1

Research Article

Paraoxon Attenuates Vascular Smooth Muscle Contraction through Inhibiting Ca2+ Influx in the Rabbit Thoracic Aorta

Figure 1

Paraoxon attenuated phenylephrine- (Phe-) or KCl-induced vasoconstriction in rabbit thoracic aorta. The effect of pretreatment with paraoxon on phenylephrine- (a) or KCl- (b) induced contraction in rabbits thoracic aorta in the presence or absence of aortic endothelium. The aortic ring preparations were preincubated with (30  πœ‡ M) or without (vehicle) paraoxon for 30 minutes followed by stimulation with Phe (1  πœ‡ M) or KCl (80 mM). In some ring preparations with aortic endothelium (Endo), L-NNA (30  πœ‡ M) was incubated during Phe-induced contraction. In some other ring preparations without aortic endothelium, sodium nitroprusside (SNP: 1 nM) and atropine (5 πœ‡ M) were incubated during Phe-induced contraction in the absence of paraoxon. (c) Concentration-response curves for Phe-induced vasoconstriction of endothelium-denuded aortic rings in the presence (0.3 πœ‡ M–30 πœ‡ M) or absence (vehicle) of pretreatment with paraoxon (30 πœ‡ M) for 30 minutes. (d) Concentration-response curves for KCl-induced vasoconstriction of endothelium-denuded aortic rings in the presence (0.3 πœ‡ M–30 πœ‡ M) or absence (vehicle) of paraoxon (pre-treatment for 30 min). Each contractile value was standardized against the contraction level induced by 50 mM KCl in the absence of pretreatment. Data are expressed as means Β± SEM (n = 8). βˆ— 𝑃 < . 0 5 ; βˆ— 𝑃 < . 0 5 compared with the vehicle group.
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