Proposing Low-Similarity Peptide Vaccines against Mycobacterium tuberculosis
Figure 2
The immunogenic impact of a single amino acid substitution in the five core residues (AGNVN, aa 71–75) of the immunodominant p61–80/PT19 mycobacterial epitope (VTGSVVCTTAAGNVNIAIGG). The substitution of N73A impairs T immunogenicity for the target epitope and changes the proteomic similarity level of the five core residues (aa 71–75 underlined) from one match (aa sequence AGNVN) to eight matches (aa sequence AGAVN). (a) The immunogenic MHC-peptide complex containing the central low-similarity pentapeptide AGNVN (in white); (b) The nonimmunogenic MHC-peptide complex containing the higher similarity pentapeptide AGAVN (in gray). Immunoreactivity and mutagenesis experiments are described in [58]. The figures are modified versions of the original drawings from the website http://www.iayork.com/ by kind authorization of Professor Ian York, University of Michigan.
