Tomatine Adjuvantation of Protective Immunity to a Major Pre-erythrocytic Vaccine Candidate of Malaria is Mediated via T Cell Release of IFN-
Cytolytic activity of splenocytes from mice immunized with adjuvant-antigen preparations of tomatine-P. berghei CS peptide or tomatine-saline following ex vivo restimulation with P. berghei CS peptide (25 g/mL). Control cytolytic activity of similarly stimulated splenocytes from naive mice is also shown. Data represent percentage specific lysis of P815 target cells loaded with P. berghei CS peptide or with no peptide (medium control) over a range of effector: target cell ratios. Data shown represent the mean of four mice, assayed individually in triplicate, from one of four similar experiments. Cytolytic activity following CS peptide stimulation (shown in bold): versus tomatine-saline group, versus naive group, versus tomatine-saline group, determined by the Mann-Whitney U-test. The positive control cytolytic T cell clone CS.C7 specific for P. berghei CS peptide elicited 81.2 and 53.0% P815 cell lysis at an effector: target ratio of 100 : 1 and 50 : 1, respectively.
Percentage target cell lysis
Effector: target cell ratio
100 : 1
50 : 1
25 : 1
1 : 1
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