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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 847673, 9 pages
Research Article

Size of Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels

1Genetics and Plant Breeding, Maringá State University (UEM), Colombo Avenue 5790, Paraná Maringá 87020-900, Brazil
2Biotechnology laboratory, Embrapa Soybean, Carlos João Strass road, Warta County, Paraná Londrina 86001-970, Brazil
3Biology Department, Filadelfia University, Juscelino Kubitschek, Avenue 1626, Paraná Londrina 86020-000, Brazil

Received 2 September 2009; Accepted 13 December 2009

Academic Editor: Jose Botella

Copyright © 2010 Renata Fuganti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


During earlier experiments, an SSR molecular marker (176 Soy HSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between and in size and susceptible genotypes, . Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more insertions. These results suggest that the number of insertions inside this promoter region could modulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of insertions.