BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules: Molecular Cloning, Preparation, and In Vitro Analysis
Figure 1
Schematic representation of molecular cloning strategy for generation of recombinant BacMam virus and microencapsulation of transduced cells. The reporter gene hMGFP was excised along with the CMV promoter from phMGFP vector using BglII and XbaI restriction enzymes and inserted in the MCS of the baculovirus transfer vector pVL1392 to construct the PCMV-hMGFP/pVL1392 plasmid. The BacMam viruses generated by cotransfection were transduced to the cardiomyocytes. This was followed by microencapsulation and tracking the MGFP expression of the transduced cells.
