BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules: Molecular Cloning, Preparation, and In Vitro Analysis
Figure 7
Immunoprotective potential of the AP-PEG-A membrane. Microcapsules were prepared by microencapsulating Bac-null transduced H9c2 cells/ml of alginate and cultured in DMEM+10% FBS in 37°C incubator for 8 days in presence of lymphocytes. Medium was replaced on every alternate day. Samples were collected at regular intervals and subjected to viability test. Viability tests were done by using the calcein AM (showing viable cells in green) and ethidium homopolymer (showing dead cells in red) dye. The data represent the mean of three independent studies, and error bars represent standard error of the mean (a). Representative bright field and fluorescent images of a capsule encapsulating the cells under 200x magnifications in a fluorescence microscope (b). The white-dotted circles show the peripheral surface of the capsules.
