Figure 3: Effect of rSj16 on adherence and morphology of WEHI-3B JCS cells. WEHI-3B JCS cells were treated with 0.5  𝜇 g/mL rSj16 (rSj16), 0.5  𝜇 g/mL rGST (rGST), or medium alone (untreated) for various time periods, and morphological changes were studies using a light microscope (Nikon). (a) Time course morphological changes of WEHI-3B JCS cells (magnification, × 200). Black bars represent 50 𝜇 m. (b) Induction of adherence of WEHI-3B JCS cells after exposure to rSj16. The data were expressed as mean ± SEM, 𝑛 = 3 . 𝑃 < . 0 1 ; 𝑃 < . 0 0 1 as compared with that of untreated group. (c)–(e) Modified Wright Giemsa staining of WEHI-3B JCS cells 72 hours post treatment (magnification, × 400). Black bars represent 25  𝜇 m. Data are representative of three individual experiments.