Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages
Figure 3
Effect of rSj16 on adherence and morphology of WEHI-3B JCS cells. WEHI-3B JCS cells were treated with 0.5 g/mL rSj16 (rSj16), 0.5 g/mL rGST (rGST), or medium alone (untreated) for various time periods, and morphological changes were studies using a light microscope (Nikon). (a) Time course morphological changes of WEHI-3B JCS cells (magnification, 200). Black bars represent 50 m. (b) Induction of adherence of WEHI-3B JCS cells after exposure to rSj16. The data were expressed as mean SEM, . ; as compared with that of untreated group. (c)–(e) Modified Wright Giemsa staining of WEHI-3B JCS cells 72 hours post treatment (magnification, 400). Black bars represent 25 m. Data are representative of three individual experiments.