Figure 2: Spleen changes of mice infected with T. cruzi strains. Groups of three mice were inoculated with PBS (grey bar) or 1 × 1 0 5 T. cruzi BT of Ninoa (white bar) or Qro (black bar) strains and spleens were obtained at acute phase of infection. (a) Growth of spleen from mice infected with Ninoa (center) and Qro (right) strains compared with control non infected mice (left). Size (b) and Weight (c) of spleen from mice infected. Media value and standard deviation are shown. Statistical differences ( 𝑃 < . 0 0 1 ) were determined between control and infected mice ( ) and between Ninoa and Qro strains ( ) by ANOVA Bonferroni post-test.