Figure 6: mRNA expression of MIG (CXCL9), MCP-1 (CCL2), MIP-1β (CCL4), and Rantes (CCL5) in cardiac tissue of Balb/c mice infected with 1 × 1 0 5 BT of T. cruzi. Total RNA was obtained at day 21 post infection and chemokines expression was determined by RT-PCR as described in material and methods. PCR products were analyzed in 2% agarose gels stained with ethidium bromide. Semicuantitative analysis was done. Values are average of 4 mice from 3 independent assays. Grey bar control, black bar Ninoa strain and White bar Qro strain. In the case of MIG and MIP-1β no detectable levels of chemokines were found in the control tissues. Significant differences ( 𝑃 < . 0 5 ) in chemokine expression were found in cardiac tissue of control versus infected mice. Differences between T. cruzi strains were also founded ( 𝑃 < . 0 5 ) .