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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 906082, 15 pages
http://dx.doi.org/10.1155/2010/906082
Review Article

Challenges for Biomarker Discovery in Body Fluids Using SELDI-TOF-MS

1Laboratory of Clinical Chemistry, GIGA Research, University of Liège, CHU, B36, 4000 Liège 1, Belgium
2Department of Rheumatology, GIGA Research, University of Liège, CHU, B36, 4000 Liège 1, Belgium
3Proteomic Platform, GIGA Research, University of Liège, CHU, B36, 4000 Liège 1, Belgium
4Department of Hepato-Gastroenterology, GIGA Research, University of Liège, CHU, B36, 4000 Liège 1, Belgium

Received 27 June 2009; Accepted 1 September 2009

Academic Editor: Pieter C. Dorrestein

Copyright © 2010 Muriel De Bock et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Protein profiling using SELDI-TOF-MS has gained over the past few years an increasing interest in the field of biomarker discovery. The technology presents great potential if some parameters, such as sample handling, SELDI settings, and data analysis, are strictly controlled. Practical considerations to set up a robust and sensitive strategy for biomarker discovery are presented. This paper also reviews biological fluids generally available including a description of their peculiar properties and the preanalytical challenges inherent to sample collection and storage. Finally, some new insights for biomarker identification and validation challenges are provided.