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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 907964, 11 pages
http://dx.doi.org/10.1155/2010/907964
Research Article

Induction by TNF- of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads

1BioPharmaNet, Department of Biochemistry and Molecular Biology, Ferrara University, 44121 Ferrara, Italy
2CAPS-IT, Department of Chemistry and Technology of Drugs, University of Perugia, 06123 Perugia, Italy

Received 23 March 2010; Revised 21 June 2010; Accepted 28 June 2010

Academic Editor: Douglas Cyr

Copyright © 2010 Monica Borgatti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have developed a microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells. The applied method is based on generation of monodisperse droplets by a vibrational nozzle. Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath. We have found that the microencapsulation procedure does not alter the viability of the encapsulated IB3-1 cells. The encapsulated IB3-1 cells were characterized in term of secretomic profile, analyzing the culture medium by Bio-Plex strategy. The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent. In order to determine the biotechnological applications of this procedure, we determined whether encapsulated IB3-1 cells could be induced to pro-inflammatory responses, after treatment with TNF- . In this experimental set-up, encapsulated and free IB3-1 cells were treated with TNF- , thereafter the culture media from both cell populations were collected. As expected, TNF- induced a sharp increase in the secretion of interleukins, chemokines and growth factors. Of great interest was the evidence that induction of interleukin-6 and interleukin-8 occurs also by encapsulated IB3-1 cells.