Methodology Report

A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation

Figure 4

1DGE analysis of human blood plasma before and after prefractionation using immunoaffinity depletion of high-abundant proteins or hexapeptide ligand library treatment. An equal amount of proteins from whole plasma (lane 1), plasma fraction from MARS-Hu 14 (lane 2), plasma fraction from ProteoMiner (lane 3), and potentially remaining proteins from boiled in SDS loading buffer ProteoMiner beads after elution (lane 4) were separated on 12.5% SDS-PAGE gel and were stained with Deep Purple.