BioMed Research International / 2010 / Article / Tab 2

Methodology Report

A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation

Table 2

Some of the weakly abundant plasma proteins identified with two or more peptides after immunoaffinity depletion of high-abundant proteins (IM) or hexapeptide ligand library treatment (PM). Associated concentrations were found in the literature in the mid-range from 5 to 50 000 ng/mL serum or plasma.

Protein namePrefractionationConcentrationBibliographic reference

Plasma retinol-binding proteinPM44.4  πœ‡ g/mL (plasma) [20]
IM
KallistatinIM22.1  πœ‡ g/mL (plasma)[21]
Ficolin-3IM21.6  πœ‡ g/mL (plasma)[22]
TetranectinIM13.75  πœ‡ g/mL (serum)[23]
Selenoprotein PIM5.3  πœ‡ g/mL (plasma)[24]
Von Willebrand factorPM1.3  πœ‡ g/mL (plasma) [16]
IM
Intelectin-1PM0.1 to 1.0  πœ‡ g/mL (serum)[25]
Extracellular SODPM150 ng/mL (plasma) [26]
IM
Mannose-binding lectin (Protein C)IM97 ng/mL (plasma)[16]
Insulin-like growth factor binding protein 3PM59 ng/mL (plasma) [16]
IM
P-selectinIM30 ng/mL (plasma)[17]
Cadherin 5PM30 ng/mL (plasma) [16]
IM
Macrophage colony-stimulating factor 1 receptor (M-CSF R)IM26 ng/mL (plasma)[16]
Hepatocyte growth factor activatorPM17.6 ng/mL (serum) [18]
IM
Insulin-like growth factor binding protein 2PM15 ng/mL (plasma) [16]
IM
Sex hormone-binding globulinPMto 8.1 ng/mL (plasma) [19]
IM