A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation

Table 2

Some of the weakly abundant plasma proteins identified with two or more peptides after immunoaffinity depletion of high-abundant proteins (IM) or hexapeptide ligand library treatment (PM). Associated concentrations were found in the literature in the mid-range from 5 to 50 000 ng/mL serum or plasma.

Protein name	Prefractionation	Concentration	Bibliographic reference
Plasma retinol-binding protein	PM	44.4 𝜇g/mL (plasma)	[20]
	IM
Kallistatin	IM	22.1 𝜇g/mL (plasma)	[21]
Ficolin-3	IM	21.6 𝜇g/mL (plasma)	[22]
Tetranectin	IM	13.75 𝜇g/mL (serum)	[23]
Selenoprotein P	IM	5.3 𝜇g/mL (plasma)	[24]
Von Willebrand factor	PM	1.3 𝜇g/mL (plasma)	[16]
	IM
Intelectin-1	PM	0.1 to 1.0 𝜇g/mL (serum)	[25]
Extracellular SOD	PM	150 ng/mL (plasma)	[26]
	IM
Mannose-binding lectin (Protein C)	IM	97 ng/mL (plasma)	[16]
Insulin-like growth factor binding protein 3	PM	59 ng/mL (plasma)	[16]
	IM
P-selectin	IM	30 ng/mL (plasma)	[17]
Cadherin 5	PM	30 ng/mL (plasma)	[16]
	IM
Macrophage colony-stimulating factor 1 receptor (M-CSF R)	IM	26 ng/mL (plasma)	[16]
Hepatocyte growth factor activator	PM	17.6 ng/mL (serum)	[18]
	IM
Insulin-like growth factor binding protein 2	PM	15 ng/mL (plasma)	[16]
	IM
Sex hormone-binding globulin	PM	to 8.1 ng/mL (plasma)	[19]
	IM