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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 970491, 14 pages
http://dx.doi.org/10.1155/2010/970491
Research Article

Expression and Purification of Z Protein from Junín Virus

1Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, LIGBCM, Roque Sáenz Peña 352, CP B1876BXD, Bernal, Buenos Aires, Argentina
2Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
3Unité de Virologie Structurale, Pasteur Institut, 7524 París, France

Received 18 February 2010; Revised 14 April 2010; Accepted 21 April 2010

Academic Editor: Norbert K. Herzog

Copyright © 2010 S. E. Goñi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. S. Salvato, J. C. S. Clegg, M. J. Buchmeier et al., “Family arenaviridae,” in Virus Taxonomy, Eighth Report of the International Committee on Taxonomy of Viruses, M. H. V. Van Regenmortel, C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, and L. A. Ball, Eds., Academic Press, 2005. View at Google Scholar
  2. D. D. Auperin, V. Romanowski, M. Galinski, and D. H. L. Bishop, “Sequencing studies of Pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA,” Journal of Virology, vol. 52, no. 3, pp. 897–904, 1984. View at Google Scholar · View at Scopus
  3. P. D. Ghiringhelli, R. V. Rivera-Pomar, M. E. Lozano, O. Grau, and V. Romanowski, “Molecular organization of Junin virus S RNA: complete nucleotide sequence, relationship with other members of the Arenaviridae and unusual secondary structures,” Journal of General Virology, vol. 72, no. 9, pp. 2129–2141, 1991. View at Google Scholar · View at Scopus
  4. M. J. Buchmeier, J. C. de la Torre, and C. J. Peters, “Arenaviridae: the virus and their replication,” in Fields Virology, M. Knipe and P. M. Howley, Eds., vol. 2, pp. 1791–1827, Lippincott Williams & Wilkins, Philadelphia, Pa, USA, 5th edition, 2007. View at Google Scholar
  5. R. Jácamo, N. López, M. Wilda, and M. T. Franze-Fernández, “Tacaribe virus Z protein interacts with the L polymerase protein to inhibit viral RNA synthesis,” Journal of Virology, vol. 77, no. 19, pp. 10383–10393, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Iapalucci, N. López, O. Rey, M. M. Zakin, G. N. Cohen, and M. T. Franze-Fernández, “The 5' region of Tacaribe virus L RNA encodes a protein with a potential metal binding domain,” Virology, vol. 173, no. 1, pp. 357–361, 1989. View at Google Scholar · View at Scopus
  7. R. N. Charrel, H. Feldmann, C. F. Fulhorst, R. Khelifa, R. D. Chesse, and X. D. Lamballerie, “Phylogeny of New World arenaviruses based on the complete coding sequences of the small genomic segment identified an evolutionary lineage produced by intrasegmental recombination,” Biochemical and Biophysical Research Communications, vol. 296, no. 5, pp. 1118–1124, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. M. S. Salvato, K. J. Schweighofer, J. Burns, and E. M. Shimomaye, “Biochemical and immunological evidence that the 11 kDa zinc-binding protein of lymphocytic choriomeningitis virus is a structural component of the virus,” Virus Research, vol. 22, no. 3, pp. 185–198, 1992. View at Publisher · View at Google Scholar · View at Scopus
  9. K. L. B. Borden, E. J. Campbell Dwyer, and M. S. Salvato, “An arenavirus RING (zinc-binding) protein binds the oncoprotein promyelocyte leukemia protein (PML) and relocates PML nuclear bodies to the cytoplasm,” Journal of Virology, vol. 72, no. 1, pp. 758–766, 1998. View at Google Scholar · View at Scopus
  10. K. L. B. Borden, E. J. Campbelldwyer, G. W. Carlile, M. Djavani, and M. S. Salvato, “Two RING finger proteins, the oncoprotein PML and the arenavirus Z protein, colocalize with the nuclear fraction of the ribosomal P proteins,” Journal of Virology, vol. 72, no. 5, pp. 3819–3826, 1998. View at Google Scholar · View at Scopus
  11. E. J. Campbell Dwyer, H. Lai, R. C. MacDonald, M. S. Salvato, and K. L. B. Borden, “The lymphocytic choriomeningitis virus RING protein Z associates with eukaryotic initiation factor 4E and selectively represses translation in a RING-dependent manner,” Journal of Virology, vol. 74, no. 7, pp. 3293–3300, 2000. View at Publisher · View at Google Scholar · View at Scopus
  12. A. Kentsis, E. C. Dwyer, J. M. Perez et al., “The RING domains of the promyelocytic leukemia protein PML and the arenaviral protein Z repress translation by directly inhibiting translation initiation factor eIF4E,” Journal of Molecular Biology, vol. 312, no. 4, pp. 609–623, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. D. Garcin, S. Rochat, and D. Kolakofsky, “The Tacaribe arenavirus small zinc finger protein is required for both mRNA synthesis and genome replication,” Journal of Virology, vol. 67, no. 2, pp. 807–812, 1993. View at Google Scholar · View at Scopus
  14. N. López, R. Jácamo, and M. T. Franze-fernández, “Transcription and RNA replication of tacaribe virus genome and antigenome analogs require n and l proteins: Z protein is an inhibitor of these processes,” Journal of Virology, vol. 75, no. 24, pp. 12241–12251, 2001. View at Publisher · View at Google Scholar · View at Scopus
  15. M. Pérez, R. C. Craven, and J. C. de la Torre, “The small RING finger protein Z drives arenavirus budding: implications for antiviral strategies,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 22, pp. 12978–12983, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. E. O. Freed, “Viral late domains,” Journal of Virology, vol. 76, no. 10, pp. 4679–4687, 2002. View at Publisher · View at Google Scholar · View at Scopus
  17. O. Pornillos, J. E. Garrus, and W. I. Sundquist, “Mechanisms of enveloped RNA virus budding,” Trends in Cell Biology, vol. 12, no. 12, pp. 569–579, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. J. Martin-Serrano, D. Pérez-Caballero, and P. D. Bieniasz, “Context-dependent effects of L domains and ubiquitination on viral budding,” Journal of Virology, vol. 78, no. 11, pp. 5554–5563, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. P. D. Bieniasz, “Late budding domains and host proteins in enveloped virus release,” Virology, vol. 344, no. 1, pp. 55–63, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Urata, T. Noda, Y. Kawaoka, H. Yokosawa, and J. Yasuda, “Cellular factors required for Lassa virus budding,” Journal of Virology, vol. 80, no. 8, pp. 4191–4195, 2006. View at Publisher · View at Google Scholar · View at Scopus
  21. S. E. Goñi, J. A. Iserte, A. M. Ambrosio, V. Romanowski, P. D. Ghiringhelli, and M. E. Lozano, “Genomic features of attenuated Junín virus vaccine strain candidate,” Virus Genes, vol. 32, no. 1, pp. 37–41, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. A. S. Parodi, D. J. Greenway, H. R. Rugiero et al., “Sobre la etiología del brote epidémico en Junín,” Día Médico, vol. 30, pp. 2300–2302, 1958. View at Google Scholar
  23. J. L. Vaughn, R. H. Goodwin, G. J. Tompkins, and P. McCawley, “The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae),” In Vitro, vol. 13, no. 4, pp. 213–217, 1977. View at Google Scholar · View at Scopus
  24. R. R. Granados, L. Guoxun, A. C. G. Derksen, and K. A. McKenna, “A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus,” Journal of Invertebrate Pathology, vol. 64, no. 3, pp. 260–266, 1994. View at Publisher · View at Google Scholar · View at Scopus
  25. S. E. Luria and J. W. Burrous, “Hybridization between Escherichia coli and Shigella,” The Journal of Bacteriology, vol. 74, pp. 461–476, 1957. View at Google Scholar
  26. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  27. T. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA, 1st edition, 1982.
  28. L. Volpon, M. J. Osborne, and K. L. Borden, “NMR assignment of the arenaviral protein Z from Lassa fever virus,” Biomolecular NMR Assignments, vol. 2, no. 1, pp. 81–84, 2008. View at Publisher · View at Google Scholar · View at Scopus
  29. G. A. Villegas, M. H. Argüelles, A. A. Castello, N. J. Mas, and G. Glikmann, “A rapid method to produce high yields of purified rotavirus particles,” Journal of Virological Methods, vol. 104, no. 1, pp. 9–19, 2002. View at Publisher · View at Google Scholar · View at Scopus
  30. M. H. Argüelles, M. L. Orellana, A. A. Castello et al., “Measles virus-specific antibody levels in individuals in Argentina who received a one-dose vaccine,” Journal of Clinical Microbiology, vol. 44, no. 8, pp. 2733–2738, 2006. View at Publisher · View at Google Scholar · View at Scopus
  31. J. D. Thompson, T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins, “The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools,” Nucleic Acids Research, vol. 25, no. 24, pp. 4876–4882, 1997. View at Publisher · View at Google Scholar · View at Scopus
  32. J. D. Thompson, D. G. Higgins, and T. J. Gibson, “CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Research, vol. 22, no. 22, pp. 4673–4680, 1994. View at Google Scholar · View at Scopus
  33. G. E. Crooks, G. Hon, J.-M. Chandonia, and S. E. Brenner, “WebLogo: a sequence logo generator,” Genome Research, vol. 14, no. 6, pp. 1188–1190, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. T. D. Schneider and R. M. Stephens, “Sequence logos: a new way to display consensus sequences,” Nucleic Acids Research, vol. 18, no. 20, pp. 6097–6100, 1990. View at Google Scholar · View at Scopus
  35. N. Hulo, A. Bairoch, V. Bulliard et al., “The 20 years of PROSITE,” Nucleic Acids Research, vol. 36, no. 1, pp. D245–D249, 2008. View at Publisher · View at Google Scholar · View at Scopus
  36. E. Gasteiger, A. Gattiker, C. Hoogland, I. Ivanyi, R. D. Appel, and A. Bairoch, “ExPASy: the proteomics server for in-depth protein knowledge and analysis,” Nucleic Acids Research, vol. 31, no. 13, pp. 3784–3788, 2003. View at Publisher · View at Google Scholar · View at Scopus
  37. S. Maurer-Stroh and F. Eisenhaber, “Myristoylation of viral and bacterial proteins,” Trends in Microbiology, vol. 12, no. 4, pp. 178–185, 2004. View at Publisher · View at Google Scholar · View at Scopus
  38. J. C. Casabona, J. M. Levingston Macleod, M. E. Loureiro, G. A. Gomez, and N. Lopez, “The RING domain and the L79 residue of Z protein are involved in both the rescue of nucleocapsids and the incorporation of glycoproteins into infectious chimeric arenavirus-like particles,” Journal of Virology, vol. 83, no. 14, pp. 7029–7039, 2009. View at Publisher · View at Google Scholar · View at Scopus
  39. O. Pornillos, S. L. Alam, D. R. Davis, and W. I. Sundquist, “Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein,” Nature Structural Biology, vol. 9, no. 11, pp. 812–817, 2002. View at Google Scholar · View at Scopus
  40. S. Lee, A. Joshi, K. Nagashima, E. O. Freed, and J. H. Hurley, “Structural basis for viral late-domain binding to Alix,” Nature Structural and Molecular Biology, vol. 14, no. 3, pp. 194–199, 2007. View at Publisher · View at Google Scholar · View at Scopus
  41. S. Urata, J. Yasuda, and J. C. De La Torre, “The Z protein of the new world arenavirus tacaribe virus has bona fide budding activity that does not depend on known late domain motifs,” Journal of Virology, vol. 83, no. 23, pp. 12651–12655, 2009. View at Publisher · View at Google Scholar · View at Scopus
  42. A. P. Schmitt, G. P. Leser, E. Morita, W. I. Sundquist, and R. A. Lamb, “Evidence for a new viral late-domain core sequence, FPIV, necessary for budding of a paramyxovirus,” Journal of Virology, vol. 79, no. 5, pp. 2988–2997, 2005. View at Publisher · View at Google Scholar · View at Scopus
  43. A. L. Demain and P. Vaishnav, “Production of recombinant proteins by microbes and higher organisms,” Biotechnology Advances, vol. 27, no. 3, pp. 297–306, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. A. Kentsis, R. E. Gordon, and K. L. B. Borden, “Self-assembly properties of a model RING domain,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 2, pp. 667–672, 2002. View at Publisher · View at Google Scholar · View at Scopus
  45. K. Hayashi and C. Kojima, “pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production,” Protein Expression and Purification, vol. 62, no. 1, pp. 120–127, 2008. View at Publisher · View at Google Scholar · View at Scopus
  46. T. Strecker, R. Eichler, J. Ter Meulen et al., “Lassa virus Z protein is a matrix protein sufficient for the release of virus-like particles,” Journal of Virology, vol. 77, no. 19, pp. 10700–10705, 2003. View at Publisher · View at Google Scholar · View at Scopus
  47. T. Strecker, A. Maisa, S. Daffis, R. Eichler, O. Lenz, and W. Garten, “The role of myristoylation in the membrane association of the Lassa virus matrix protein Z,” Virology Journal, vol. 3, article 93, 2006. View at Publisher · View at Google Scholar · View at Scopus
  48. A. A. Capul, M. Perez, E. Burke, S. Kunz, M. J. Buchmeier, and J. C. De La Torre, “Arenavirus Z-glycoprotein association requires Z myristoylation but not functional RING or late domains,” Journal of Virology, vol. 81, no. 17, pp. 9451–9460, 2007. View at Publisher · View at Google Scholar · View at Scopus
  49. M. Pérez, D. L. Greenwald, and J. C. De La Torre, “Myristoylation of the RING finger Z protein is essential for arenavirus budding,” Journal of Virology, vol. 78, no. 20, pp. 11443–11448, 2004. View at Publisher · View at Google Scholar · View at Scopus
  50. K. L. Warfield, N. A. Posten, D. L. Swenson et al., “Filovirus-like particles produced in insect cells: immunogenicity and protection in rodents,” Journal of Infectious Diseases, vol. 196, no. 2, pp. S421–S429, 2007. View at Publisher · View at Google Scholar · View at Scopus