Table of Contents Author Guidelines Submit a Manuscript
Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 971340, 5 pages
http://dx.doi.org/10.1155/2010/971340
Research Article

Novel Method of Cell-Free In Vitro Synthesis of the Human Fibroblast Growth Factor 1 Gene

Orthodontics, Faculty of Dentistry, The University of Hong Kong, 2/F, Prince Philip Dental Hospital, 34 Hospital Road, SAR, Hong Kong

Received 7 May 2010; Accepted 16 June 2010

Academic Editor: G. S. Stein

Copyright © 2010 Peijun Zuo and A. Bakr M. Rabie. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. K. Jayaraman and C. J. Puccini, “A PCR-mediated gene synthesis strategy involving the assembly of oligonucleotides representing only one of the strands,” BioTechniques, vol. 12, no. 3, pp. 392–398, 1992. View at Google Scholar · View at Scopus
  2. K. Jayaraman, S. A. Fingar, J. Shah, and J. Fyles, “Polymerase chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme c of horseradish peroxidase,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 10, pp. 4084–4088, 1991. View at Google Scholar · View at Scopus
  3. K. Jayaraman, J. Shah, and J. Fyles, “PRC mediated gene synthesis,” Nucleic Acids Research, vol. 17, no. 11, p. 4403, 1989. View at Google Scholar · View at Scopus
  4. R. C. Scarpulla, S. Narang, and R. Wu, “Use of a new retrieving adaptor in the cloning of a synthetic human insulin A-chain gene,” Analytical Biochemistry, vol. 121, no. 2, pp. 356–365, 1982. View at Google Scholar · View at Scopus
  5. N. K. Gupta, E. Ohtsuka, V. Sgaramella et al., “Studies on polynucleotides, 88. Enzymatic joining of chemically synthesized segments corresponding to the gene for alanine-tRNA,” Proceedings of the National Academy of Sciences of the United States of America, vol. 60, no. 4, pp. 1338–1344, 1968. View at Google Scholar · View at Scopus
  6. W. Mandecki and T. J. Bolling, “FokI method of gene synthesis,” Gene, vol. 68, no. 1, pp. 101–107, 1988. View at Google Scholar · View at Scopus
  7. F. Barany, “Genetic disease detection and DNA amplification using cloned thermostable ligase,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 1, pp. 189–193, 1991. View at Publisher · View at Google Scholar · View at Scopus
  8. W. P. C. Stemmer, “Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides,” Gene, vol. 164, no. 1, pp. 49–53, 1995. View at Publisher · View at Google Scholar · View at Scopus
  9. R. B. Ciccarelli, P. Gunyuzlu, J. Huang, C. Scott, and F. T. Oakes, “Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands,” Nucleic Acids Research, vol. 19, no. 21, pp. 6007–6013, 1991. View at Google Scholar · View at Scopus
  10. A. S. Xiong, Q. H. Yao, R. H. Peng et al., “A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences,” Nucleic Acids Research, vol. 32, no. 12, article e98, 2004. View at Google Scholar · View at Scopus
  11. L. Young and Q. Dong, “Two-step total gene synthesis method,” Nucleic Acids Research, vol. 32, no. 7, article e59, 2004. View at Google Scholar · View at Scopus
  12. S. R. Thaker, S. K. Dutta, S. L. Adhya, and B. L. Mattingly-Napier, “Molecular cloning of Ehrlichia risticii development of a gene probe for the diagnosis of potomac horse fever,” Journal of Clinical Microbiology, vol. 28, no. 9, pp. 1963–1967, 1990. View at Google Scholar · View at Scopus
  13. P. Zuo and B. M. Rabie, “One-step DNA fragment assembly and circularization for gene cloning,” Current Issues in Molecular Biology, vol. 12, no. 1, pp. 11–16, 2009. View at Google Scholar
  14. M. Csurös, I. B. Rogozin, and E. V. Koonin, “Extremely intron-rich genes in the alveolate ancestors inferred with a flexible maximum-likelihood approach,” Molecular Biology and Evolution, vol. 25, no. 5, pp. 903–911, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. T. Yoneyama, H. Kasuya, H. Onda et al., “Association of positional and functional candidate genes FGF1, FBN2, and LOX on 5q31 with intracranial aneurysm,” Journal of Human Genetics, vol. 48, no. 6, pp. 309–314, 2003. View at Google Scholar · View at Scopus
  16. F. L. Graham, J. Smiley, W. C. Russell, and R. Nairn, “Characteristics of a human cell line transformed by DNA from human adenovirus type 5,” Journal of General Virology, vol. 36, no. 1, pp. 59–72, 1977. View at Google Scholar · View at Scopus
  17. J. C. Lee and R. R. Gutell, “Diversity of base-pair conformations and their occurrence in rRNA structure and RNA structural motifs,” Journal of Molecular Biology, vol. 344, no. 5, pp. 1225–1249, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. A. Telesnitsky, S. Blain, and S. P. Goff, “Assays for retroviral reverse transcriptase,” Methods in Enzymology, vol. 262, pp. 347–362, 1995. View at Publisher · View at Google Scholar · View at Scopus
  19. E. V. Koonin, T. G. Senkevich, and V. V. Dolja, “Compelling reasons why viruses are relevant for the origin of cells,” Nature Reviews Microbiology, vol. 7, no. 8, p. 615, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. R. Rappuoli, V. Scarlato, V. Masignani et al., “Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing,” Science, vol. 287, no. 5459, pp. 1816–1820, 2000. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Aguzzi, “Unraveling prion strains with cell biology and organic chemistry,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 1, pp. 11–12, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. C. B. Anfinsen, “The formation and stabilization of protein structure,” Biochemical Journal, vol. 128, no. 4, pp. 737–749, 1972. View at Google Scholar · View at Scopus
  23. M. C. Wolf, Y. Wang, A. N. Freiberg, H. C. Aguilar, M. R. Holbrook, and B. Lee, “A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics,” Virology Journal, vol. 6, article 119, 2009. View at Publisher · View at Google Scholar · View at Scopus