Table of Contents Author Guidelines Submit a Manuscript
Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 980567, 12 pages
http://dx.doi.org/10.1155/2010/980567
Research Article

Constitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli

Center for Bioindustrial Technology, Agency for Assessment and Application of Technology (BPPT), Jl MH Thamrin no. 8, Jakarta 10340, Indonesia

Received 22 February 2010; Revised 21 May 2010; Accepted 6 July 2010

Academic Editor: Daniele Daffonchio

Copyright © 2010 Is Helianti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. Muksin, “Pengolahan material serat alami menggunakan enzim mikrobiologi untuk media ekspresi seni dua dimensi,” Journal of Visual Art, vol. 1, pp. 401–416, 2007, (Indonesian). View at Google Scholar
  2. N. Kulkarni, A. Shendye, and M. Rao, “Molecular and biotechnological aspects of xylanases,” FEMS Microbiology Reviews, vol. 23, no. 4, pp. 411–456, 1999. View at Publisher · View at Google Scholar · View at Scopus
  3. Q. K. Beg, M. Kapoor, L. Mahajan, and G. S. Hoondal, “Microbial xylanases and their industrial applications: a review,” Applied Microbiology and Biotechnology, vol. 56, no. 3-4, pp. 326–338, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. M. J. Vázquez, J. L. Alonso, H. Domínguez, and J. C. Parajó, “Xylooligosaccharides: manufacture and applications,” Trends in Food Science and Technology, vol. 11, no. 11, pp. 387–393, 2001. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Subramaniyan and P. Prema, “Biotechnology of microbial xylanases: enzymology, molecular biology, and application,” Critical Reviews in Biotechnology, vol. 22, no. 1, pp. 33–64, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. B. Henrissat, “A classification of glycosyl hydrolases based on amino acid sequence similarities,” Biochemical Journal, vol. 280, no. 2, pp. 309–316, 1991. View at Google Scholar · View at Scopus
  7. A. J. Oakley, T. Heinrich, C. A. Thompson, and M. C. J. Wilce, “Characterization of a family 11 xylanase from Bacillus subtillis B230 used for paper bleaching,” Acta Crystallographica D, vol. 59, no. 4, pp. 627–636, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. I. Helianti, N. Nurhayati, and B. Wahyuntari, “Cloning, sequencing, and expression of a β-1,4-endoxylanase gene from Indonesian Bacillus licheniformis strain I5 in Escherichia coli,” World Journal of Microbiology and Biotechnology, vol. 24, no. 8, pp. 1273–1279, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. T. H. Lee, P. O. Lim, and Y.-E. Lee, “Cloning, characterization, and expression of xylanase A gene from Paenibacillus sp. DG-22 in Escherichia coli,” Journal of Microbiology and Biotechnology, vol. 17, no. 1, pp. 29–36, 2007. View at Google Scholar · View at Scopus
  10. J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 3rd edition, 2001.
  11. J. D. Thompson, D. G. Higgins, and T. J. Gibson, “CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Research, vol. 22, no. 22, pp. 4673–4680, 1994. View at Google Scholar · View at Scopus
  12. J. Huang, G. Wang, and L. Xiao, “Cloning, sequencing and expression of the xylanase gene from a Bacillus subtilis strain B10 in Escherichia coli,” Bioresource Technology, vol. 97, no. 6, pp. 802–808, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Google Scholar · View at Scopus
  14. G. L. Miller, “Use of dinitrosalycylic acid as reagent for the determination of reducing sugars,” Analytical Chemistry, vol. 31, pp. 208–218, 1959. View at Google Scholar
  15. M. J. Bailey, P. Biely, and K. Poutanen, “Interlaboratory testing of methods for assay of xylanase activity,” Journal of Biotechnology, vol. 23, no. 3, pp. 257–270, 1992. View at Publisher · View at Google Scholar · View at Scopus
  16. J. G. Holt et al., Ed., Bergey’s Manual of Determinative Bacteriology, William & Wilkins, Baltimore, Md, USA, 1994.
  17. N. A. Logan and R. C. W. Berkeley, “Identification of Bacillus strains using the API system,” Journal of General Microbiology, vol. 130, no. 7, pp. 1871–1882, 1984. View at Google Scholar · View at Scopus
  18. K. S. Blackwood, C. Y. Turenne, D. Harmsen, and A. M. Kabani, “Reassessment of sequence-based targets for identification of Bacillus species,” Journal of Clinical Microbiology, vol. 42, no. 4, pp. 1626–1630, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. L. K. Nakamura, M. S. Roberts, and F. M. Cohan, “Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov,” International Journal of Systematic Bacteriology, vol. 49, no. 3, pp. 1211–1215, 1999. View at Google Scholar · View at Scopus
  20. S. Porwal, S. Lal, S. Cheema, and V. C. Kalia, “Phylogeny in aid of the present and novel microbial lineages: diversity in Bacillus,” PLoS One, vol. 4, no. 2, article e4438, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. K. Miyazaki, M. Takenouchi, H. Kondo, N. Noro, M. Suzuki, and S. Tsuda, “Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution,” Journal of Biological Chemistry, vol. 281, no. 15, pp. 10236–10242, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. R. Ruller, J. C. Rosa, V. M. Faça, L. J. Greene, and R. J. Ward, “Efficient constitutive expression of Bacillus subtilis xylanase A in Escherichia coli DH5α under the control of the Bacillus BsXA promoter,” Biotechnology and Applied Biochemistry, vol. 43, no. 1, pp. 9–15, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. M. Yamabhai, S. Emrat, S. Sukasem, P. Pesatcha, N. Jaruseranee, and B. Buranabanyat, “Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia coli expression systems,” Journal of Biotechnology, vol. 133, no. 1, pp. 50–57, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. H. Tjalsma, A. Bolhuis, J. D. H. Jongbloed, S. Bron, and J. M. van Dijl, “Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome,” Microbiology and Molecular Biology Reviews, vol. 64, no. 3, pp. 515–547, 2000. View at Google Scholar · View at Scopus