The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
Table 2
Summary of the quantitative pcr data on microcosm biofilms grown in CDFF for 8 days. The data are average log CFU-equivalents and standard deviations (SD) from 6 samples obtained from 2 CDFF runs per treatment. CHX—chlorhexidine digluconate; MLMW—mushroom low molecular weight fraction; SF4—subfraction nr 4 of MLMW fraction; SF5—subfraction nr 5 of MLMW fraction.
Negative control (water) and the respective treatments
Universal probe log CFU (SD)
S. sanguinis log CFU (SD)
V. dispar log CFU (SD)
N. subflava log CFU (SD)
F. nucleatum log CFU (SD)
A. naeslundii log CFU (SD)
P. intermedia log CFU (SD)
L. casei log CFU (SD)
S. mutans log CFU (SD)
Other bacteria$ log CFU (SD)
Water (CHX)
6.5
4.3
4.1
2.4
3.8
n.d.
n.d.
0.6
3.5
6.5
(0.3)
(0.5)
(0.5)
(0.4)
(0.5)
(0)
(0.5)
(0.3)
CHX
5.9*
3.3*
3.8
4.3
2.3*
n.d.
n.d.
0.9
n.d.*
5.8
(0.6)
(0.8)
(1.0)
(1.7)
(1.0)
(0)
(0.7)
Water (MLMW)
6.8
4.6
5.2
4.7
5.0
2.3
0.3
2.0
3.6
6.7
(0.2)
(0.4)
(0.8)
(0.4)
(0.8)
(1.3)
(0)
(0.8)
(0.9)
(0.3)
MLMW
7.0
4.5
5.8
4.5
5.8*
2.2
0.3
2.3
2.7
6.9
(0.3)
(0.6)
(0.2)
(0.8)
(0.1)
(0.6)
(0)
(0.3)
(0)
(0.3)
Water (SF4)
6.7
4.4
5.0
4.6
4.6
2.7
n.d.
2.1
4.4
6.7
(0.3)
(0.7)
(0.8)
(0.6)
(0.8)
(0)
(0.9)
(0.8)
(0.4)
SF4
6.5
4.6
5.7
5.3*
5.8*
3.2
n.d.
2.0
3.9
6.2
(0.3)
(0.4)
(0.3)
(0.2)
(0.3)
(0.9)
(0.1)
(0.4)
(0.4)
Water (SF5)
6.9
4.3
5.7
4.9
5.4
2.7
n.d.
2.6
3.4
6.7
(0.7)
(0.9)
(0.7)
(0.6)
(0.8)
(0.8)
(0.3)
(1.0)
(0.8)
SF5
6.1
4.2
5.5
4.8
5.4
2.6
n.d.
2.4
3.6
5.4
(0.6)
(0.7)
(0.5)
(0.4)
(0.5)
(0.9)
(0.2)
(0.4)
(1.0)
$Other bacteria are calculated as a difference between universal probe counts and the sum of the 8 targeted probe counts. *Statistically significantly different at from the respective water control (Mann-Whitney U test).
