A Rapid Culture Technique Produces Functional Dendritic-Like Cells from Human Acute Myeloid Leukemia Cell Lines
Figure 7
IFN-γ production from CD8+ T cells. Irradiated KG-1 (a), MUTZ-3 (b) and the derived DLC as well as FastDC and 7-day culture DC derived from PBMC (c) were cocultured at the ration of 1 : 10 for 4 days with responder PBMC loaded with CFSE. Before cells were harvested for IFN-γ assay, 10 ng/mL PMA and 200 ng/mL ionomycin were added into the culture for the last 16 hrs of incubation. Cells analysed were gated on CFSE positive CD8+ T cells. One representative result is shown.
