Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons

Figure 7

Validation of the RGA. Known amounts of IFN-β pre-determined by routine AVA were spiked into the culture media and subjected to RGA. Three expected potency ratios of the internal standard concentrations were employed, that is, 80%, 100%, and 120%. Samples were run as four replicates and potency was determined by comparing to the in-house reference standard.