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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 193052, 8 pages
Research Article

Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CN/SDS-PAGE Coupled with Mass Spectrometry

Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China

Received 16 May 2011; Revised 12 August 2011; Accepted 17 August 2011

Academic Editor: Kapil Mehta

Copyright © 2011 Zhiyun Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1 listed the infromation of identified proteins. These proteins from the protein complexes in the CN-PAGE (right, fig. 1a)were separated based on SDS-PAGE(in fig. 1b) and identified by MALDI-MS and MS/MS. Table S2 provied the information of differential expression proteins identified by MALDI-MS and MS/MS beteent SW1990 and PANC-1 cell lysate.

  1. Supplementary Tables