A Missense Mutation in Canine CLN6 in an Australian Shepherd with Neuronal Ceroid Lipofuscinosis

Table 1

PCR primer sequences used for analysis of canine the CLN6 gene.

Target	Forward primer/reverse primer	Amplicon size (bp)
Exon 1	CCGTTCGTGCTTCCCGCAAC/GGACGCGCGGTGGATGGAC	288
Exon 2	TCGAGTCCCCATACCTTTCTG/CACTGCTGGTAAGGGCTCTGTC	245
Exon 3	TTCTGGGCCTGGGTGAACAC/CTGCTTCCACTGGCCTCCATGA	243
Exon 4	GAGCCACGGCCTCCCTC/GACGGCCCGACCCACAA	267
Exon 5	TGGAGGCGCCCTGCTG/TGCGCGCTGCCCTCTAGT	281
Exon 6	CAGGACGGCTGGTGCAAG/CCAGGGCACGCACGTTT	281
Exon 7 (5′)	GTGGGTACAGCGAGGGATGCC/CCGGGTACTTCTTCCTGAGC	285
Exon 7 (3′)	CTTCGCTCTCACCCTCCTGCT/GCCGTGATGACCGCACA	249