Figure 1: Phase contrast light microscopy of tissue at 3, 10, and 17 days after explanting. (a) Cuboidal-shaped cells are clearly seen propagating from explanted tissue at day 3. (b) By day 10 cells at the periphery have a more elongated morphology (arrows). (c) At day 17, confluent areas of cells form fingerprint patterns, characteristic of fibroblast culture. Diagonal line shows scratch in Petri dish used to mince tissue and anchor tissue to culture surface. Scale bar = 25 μm.