Figure 2: Immunohistochemical staining characteristics of cells 10 days after explanting. Two distinct areas are seen—an inner most area of cuboidal-shaped cells staining for (a) cytokeratin and (b) E-cadherin (black asterisk) and stellate-shaped cells at the periphery staining for (c) the myofibroblast marker SMA. Occasional cells at the junction of the two areas stain for SMA (arrow heads). (d) All cells were negative for the endothelial marker RECA. Remnants of the tissue explant from which cells have propagated can be seen (white asterisk). Scale bar = 50 μm.