BACs as Tools for the Study of Genomic Imprinting

Table 1

Summary of BAC transgene models used to test for autonomous imprinting.

Gene (s)	Size	Imprinting (Lines imprinting/lines tested)	Expression in embryo	Reference
Nnat	270 kb BAC	1/1	Full	[1]
Nnat	95 kb BAC	2/3	Partial (lacking neural enhancers)	[1]
Cdkn1c	260 kb BAC	0/2	Full (Cdkn1c)	[2]
Cdkn1c	85 kb BAC	0/4	Partial	[2]
Peg3/Zim1	120 kb BAC	1/3(Peg3 only)	Neural expression of Peg3 but fails to rescue Peg3-deficiency	[3]
Gtl2	178 kb BAC	2/2	Partial	[4]
ZAC1/HYMAI	175 kb PAC	2/2	Full expression by in situ	[5]
Necdin and Magel	109 kb^∗ BAC	0/1	Partial (Necdin)	[6]
Igf2r	170 kb BAC	4/4	Full	[7]
Dlk1/Gtl2	70 kb BAC	0/3	Full (Dlk1)	[8]