BACs as Tools for the Study of Genomic Imprinting

Table 2

Functional consequence in transgenic overexpression models.

Gene dosage	Expressed allele	Embryonic phenotype	EE phenotype	Reference
2 X Cdkn1c; (1 copy BAC)	Maternal	E13.5 embryos 80% WT; Proportional decrease in the weight of internal organs such as kidney, lung, and liver	No placental expression	[9]
3 X Phlda2 and Slc22a18; (2 copy BAC)	Maternal	E13.5 embryos similar to WT; E16.5 embryos 90% WT weight; E18.5 embryos 87% WT	E14.5 placentae 80% WT	[10, 11]
2 X Dlk1/Gtl2; (4–7 copies BAC)	Paternal	E16.5 embryos 110% WT	No placental expression	[8]