Figure 1: Porcine myocardial characteristics. (a) Pig LV tissues electrophoresed on a large pore 1% agarose gel indicate a nearly 1 : 1 N2BA : N2B ratio. (b) N2BA/N2B ratios range from 0.2 in small animals to 1.0 in human dilated cardiomyopathy (DCM). (c) Example mechanical perturbation of pig tissue from slack SL to 2.15 μm at 10.0 lengths/s in control conditions and incubated with recombinant PEVK protein to inhibit native PEVK-actin interactions showing reduced stress relaxation (arrows). Inset: SDS-PAGE indicating the high purity of the recombinant PEVK protein fraction that was used. See text for details.