Experimentally Approaching the ICU: Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis

Figure 2

7-day mortality in 3-month-old female mice subjected to the two-hit model of trauma/hemorrhage followed by polymicrobial CLP-induced sepsis. To achieve approximately 50% mortality at post-TH day 7 (50% hemorrhage of total blood volume, TBV), the severity of CLP was adjusted by different gauge needles (20G 𝑛=10, 19G 𝑛=10, 18G 𝑛=10, 17G 𝑛=22). To reach sublethality after hemorrhage, *𝑃<.05 between 50 and 40% blood loss group (of TBV) was tested with a 17-gauge needle (40% + 17G 𝑛=65; 50% + 17G 𝑛=16).

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