Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of Pluripotent/Multipotent Stem Cells
Figure 6
In vitro culture of cells scraped from the ovarian surface epithelium. (a) Oocyte-like cell developed in vitro on autologous ovarian fibroblasts and small round cells (arrow) with diameters of 2 to 4 μm proliferating in the surroundings as attached to fibroblasts on day 4 of the culture (magnification 100-times). (b) Oocyte-like cell developed in vitro on autologous ovarian fibroblasts on day 10 of the culture (magnification 200-times). (c, d) Cell clusters developed in vitro on autologous ovarian fibroblasts on day 4 of the culture (magnification 100-times). (Inverted microscope, Hoffman illumination.) f: autologous ovarian fibroblast, o: oocyte-like cell.
