Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of Pluripotent/Multipotent Stem Cells
Figure 8
Immunocytochemical staining of cell clusters and oocyte-like cells developed during the in vitro culture of cells scraped from the ovarian surface epithelium on markers of pluripotency SOX-2 and SSEA-4. (a, b) Non-stained cell clusters (negative control) (magnifications 40-times and 100-times). (c, d). Cell clusters positively (brown) stained on SOX-2 (magnification 40-times). (e) SOX-2 non-stained round cell (negative control) (magnification 200-times). (f) Round cell positively (brown) stained on SOX-2 (magnification 200-times). (g, h) Cell clusters positively (brown) stained on SSEA-4 surface antigen (magnification 100-times). (Inverted microscope, Hoffman illumination.)
