Research Article
Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines
Table 1
Retention times of different PC-components with the same ECN on different reversed phase columns.
| | Lipid | Column type | | Nucleosil 100-5 C6H5 | Nucleosil 100-5 C18 | Nucleodur C18 Gravity | Nucleodur Sphinx RP | Nucleodur C18 Isis |
| | DPPC | 1.46 (5.76)* | 56.82 | 78.00 | 14.31 | 49.19 | | OPPC | 1.52 (6.49)* | 59.27 | 86.00 | 16.26 | 51.81 | | POPC | 1.52 (6.48)* | 57.36 | 86.00 | 16.26 | 52.19 | | DOPC | 1.64 (7.30)* | 57.18 | 98.67 | 19.11 | 56.40 | | SLPC | 1.64 (7.58)* | 69.95 | 107.00 | 19.66 | 60.81 |
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obile phase methanol/water (80 : 20).
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