Research Article

Both Basic and Acidic Amino Acid Residues of Are Involved in Triggering Substate of RyR1

Table 2

Effects of the mutant IpTxa on of purified RyR1. and for of purified RyR1 were calculated as described in the legend to Table 1. Values are means ± SE of 4–10 experiments. The average lengths of the substate events were determined at 30 nM concentration. Asterisks indicate significant differences from the wild type for each parameter (Student t-test, ). ND: not determined.

Mean duration of substate (s)
( )

Wild type0.86 ± 0.0517.08 ± 2.313.97 ± 0.18
K8A0.85 ± 0.025.29 ± 4.84*ND
R33A0.18 ± 0.06*19.25 ± 1.051.43 ± 0.09*
K30A0.16 ± 0.05*15.66 ± 5.991.88 ± 0.22*
R31A0.15 ± 0.02*18.23 ± 1.653.15 ± 0.10*