The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.)

Figure 2

BAC filter hybridization, scoring of hits, and location in fingerprint contig (FPC) map for maize CBM1.11 (umc161). A representative filter-screen hybridization (Table 2, step 2) and the autoradiograph from one of the two filters are shown (top). A total of 5 positive signals are seen (circled or boxed), and the scoring scheme for one of them (a0074A24) is illustrated at the right. The autoradiograph is labeled to show the location of the 384-well plate fields 1–6 (F1–6), and the geometry duplicate spotting of the eight individual plates per field is indicated at the right for quadrant A24. A close-up of one hit (position 2, field 3) is shown with the resulting decoded BAC ID of plate 74 position A-24 (a0074A24). This BAC, along with four other contiguous (arrows) BACs, is indicated within contig number 113 of the Sorghum propinquum FPC map (screen capture bottom left). Four additional BACs (asterisks) were detected, but not found to belong to a contig.