The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.)

Table 3

Maize CBM-RFLP identified sorghum BACs.

CBM	RFLP-probe	No. of BACS indentified	[contig] Member BACs scored	Southern blot comments
1.01	tub1	2	[868] a0039M23; [154] a0053G19	Contiguous BACs not identified
1.02	umc157	3	[154] a0045K08, a0064A06, a0055E08	All BACs confirmed
1.03	umc076	4	[193] a0046J07, a0086C09; [389] a0004B12; [?] a0071N02	BACs a0046J07, 86C09, and 71N02 confirmed
1.04	asg45	5	[490] a0073P09; [536] a0080O07; [573] a0086J05, a0075I08; [967] a0036D16	Contiguous BACs not identified
		7	[150] a0001K22; [187] a0061M20; [557] a0041B19; [?] a0029B11, a0036D16, a0043B12, a0052B23
1.05	csu003	4	[179] a0001C04, a0026E17, a0052G17, a0094G22	BACs a0001C04, 26E17, 94G22 confirmed
1.06	umc67a	2	[868] a0026H06, a0038A08
1.07	asg62	5	[550] a0084J12; [537] a0084J14; [443] a0083B09; [542] a0095N15; [567] a0096N18	Contiguous BACs not identified
1.08	umc128	12	[168] a0029P06, a0055L05, a0059H18, a0058G14, a0066G14, a0070C24, a0071B13, a0072H12, a0075H05, a0081P22, a0083A01, a0092C10	Confirmation Hybe failed; only control seen
1.09	cjd2	8	[159] a0011I10, a0023C11, a0039E21, a0056O22, a0081H24, a0091D22, a0093H06, a0093P21	BACs 23C21-93P21 confirmed
1.10	umc107a	8	[154] a0006I08, a0016J24, a0041J08, a0041O17, a0043G04, a0046O22, a0053G19, a0061G12	All BACs confirmed
1.11	umc161a	5	[147] a0014L19, a0035F09, a0058E04, a0074A24, a0092O22	All BACs confirmed; the restriction fragments of BACs 14L19 & 35F09 were smaller than those of other BACs
1.12	bnl6.32	6	[147] a0014L19, a0035F09, a0058E04; [?] a0014M19, a0067C08, a0006L16
3.01	umc32a	9	[1] a0016H11, a0020E10, a0038G18, a0049H03, a0066I03, a0083B22, a0092J15, a0092J15, a0092K15; [293] a0048H03; [?] a0035E11, a0038G18, a0015L17, a0017E12	All BACs in contig 1 confirmed
3.02	csu32	6	[1332] a0010D09, a0017F16, a0035I06, a0066B14, a0066B20; [?] a0015J10
3.03	asg24a			Contiguous BACs not identified
3.04	asg48	0		Contiguous BACs not identified
3.05	umc102	9	[898] a0016D10; [884] a0018B20; [394] a0055N13; [246] a0085B11; [894] a0083B12; [1257] a0090I03; [?] a007P18, a0012G07, a0027C12	Contiguous BACs not identified
3.06	bnl5.37a	8	[64] a0010K10, a0042I24, a0043H04, a0045D02, a0047F15, a0047J02, a0058O10, a0096N10
3.07	bnl6.16a	7 (8)	[56] a0014M18, a0020L03, a0029M08=a0029M07^∗, a0050D18, a0085M21; [143] a0051P09; [?] a0052D16
3.08	umc17a	6	[49] a0002O18, a0041K14; [85] a0074H09; [?] a0074H10, a0096D08, a0035C01
3.09	umc63a	9	[1292] a0021P11, a0025P06, a0046A02, a0043J05, a0044B15, a0055P13, a0029C04; [?] a0029C03
3.10	cyp1	6	[1118] a0029L19, a0067D01, a0078E10; [89] a0017J07, a0018N05; [?] a0096D16	All BACs in contig 1118 as well as BAC a0096D16 confirmed
4.01	agrr115a			PolyA at both ends
4.02	php20725a	10	[1] a0016H11, a0092J15, a0092K15, a0049H03; [1292] a0021P11, a0029C03, a0044B15, a0046A02, a0043J02, a0055P13	Confirmation Hybe failed
4.03	umc31a	5	[1320] a0001I16; [3] a0067O16; [?] a0057L09, a0084A23, a0007F05	Contiguous BACs not identified
4.04	npi386	7	[232] a0002B15, a0019A12, a0035B24, a0058L10, a0071D21, a0078C04, a0082H03	All BACs confirmed
4.05	agrr37b	6	[391] a0023C20, a0054H21, a0055K09, a0068N16, a0080D06, a0083G05	All BACs except 55K09 confirmed
4.06	umc156a	3	[280] a0059B12, a0061G01, a0067K14, a0089D08
4.07	umc66a			Contiguous BACs not identified
4.08	umc127c	3	[290] a0082D10, a0059L11, a0033K19	BACs 59L11 and 33K19 confirmed
4.09	umc32	6	[352] a0030M10, a0045N24, a0050L19, a0056E02, a0069D03, a0072G04
4.10	php20608	6	[313] a0008B07, a0015N19, a0019O20, a0026C03, a0063A16, a0064O16	All BACs confirmed
4.11	umc169	5	[316] a0012H04, a0017H01, a0038N14, a0040B11, a0094J01
5.01	npi409	2	[483] a0006F01; [813] a0002O16	Contiguous BACs not identified
5.02	umc90	2	[916] a0003P04; [1120] a0038C23	Contiguous BACs not identified
		4	[154] a0045K08, a0055E08, a0064A06; [?] a0034N18	All BACs confirmed
5.03	tub4	0
5.04	bnl4.36	8	[118] a0025E21, a0024H23, a0046J09, a0045L08, a0041E24, a0069P23, a0058E11, a0051H03, a0052I15; [314] a0013B03, a0061I09, a0069K04; [385] a0045M19, a0078E12, a0078A08, a0066D12	All BACs confirmed except 13B03
5.05	csu93b	7	[193] a0007P01, a0046J07, a0058O08, a0069L09, a0078B19, a0086C09; [382] a0009P04
5.06	umc125a	4	[280] a0067K14, a0061G01, a0059B12, a0089D08	All BACs confirmed
5.07	umc108	14	[287] a0095P22, a0061G08, a0063D06, a0050A23, a0052I05, a0054L12, a0046N22; [1108] a0023P17, a0004F02, a006N04; [333] a0020D11, a0029N15; [1288] a0030E06, a0038E06	All BACs in contig 287 confirmed
5.08	bnl5.24a	8	[278] a0042O01, a0020K19, a0023D21, a0095O21, a0095O19, a0081C19, a0063M08, a0002O03; [1017] a0017F09, a0017E09, a0041G23, a0019G02, a0067B22	All BACs confirmed except 63M08
5.09	php10017	6	[275] a0028A23, a0033G16, a0043E09, a0057K02, a0060N20; [?] a0061I19	All BACs confirmed except 43E09
6.01	umc085a	10	[426] a0006I03, a0009K09, a0072P24; [205] a0025E20, a0041O09, a0061M21, a0057H05, a0067N09, a0074E15, a0085D02	All BACs S. blot confirmed except 57H05
6.02	umc059a	9	[366] a0004P17, a0006P16, a0019D19, a0054G04, a0063H16, a0069J20, a0080H11, a0082K02, a0089K05	All BACs confirmed except 19D19 and 54G04
6.03	npi393	6	[759] a0004A06, a0017E10; [528] a0006D09, a0008G11, a0063A20; [?] a0039N21	All BACs confirmed
6.04	umc65a			Contiguous BACs not identified
6.05	umc21	5	[382] a0006P21, a0030G09, a0036H03, a0039E04, a0061C05
6.06	umc38a			Contiguous BACs not identified
6.07	umc132a	9	[327] a0030L20, a0040J01, a0056F09, a0060H04, a0070I02, a0070K04, a0070K07, a0073H20; [?] a0020O17	All BACs except 20O17 confirmed with cross-Hybe to negative control
6.08	asg7a	6	[323] a0018I04, a0032J21, a0032K17, a0068H17, a0084B19; [486] a0015L19	Confirmation failed
8.01	npi220a	6	[22] a0027H19, a0063B11, a0080P02, a0084E01, a0096C21; [188] a0019O09	All BACs confirmed
8.02	bnl9.11	8	[421] a0042P09, a0047M08, a0072L19, a0007N23, a0089I26=89I23^∗; [908] a0074H18; [?] a0079F24, a0035C01
8.03	umc124a	7	[6] a0014C07, a0017N03, a0040E17, a0078K17, a0002I13=a0002I16(?), a0083P05
8.04	bnl7.08a			Contiguous BACs not identified
8.05	bnl2.369			Contiguous BACs not identified
8.06	csu31a	13	[45] a0042J03, a0043C22, a0062K14, a0064A02; [233]a0089H10=a0089G10^∗; [359]a0078O19=a0078N19^∗; [915]a0085F10=; [866]77F10^∗; [?] a0035C01, a0044F14, a0053E10=51E10^∗, a0013N08, a0045C22, a0052D10,
8.07	npi268a	10	[61] a0033M15, a0073N21, a0039H13, a0045D11, a0059E23, a0065K05, a0068E24, a0072D01, a0078K07, a0089A17	Confirmation failed; only positive control seen
8.08	npi414a	9	[58] a0007I19, a0007C07, a0033M05, a0073D02,; [1019]a0002I19=a0007I19^∗; [1125] a0023G22; [223] a0046C21; [1278] a0090D06; [?] a0019G24
8.09	agrr21			Contiguous BACs not identified

* Whenever BAC scoring was ambiguous, possible alternate identified BACs were also grown for S. blot verification.