In situ PCR in local hybrid ear model and control (reproduced with modification with permission from Yamamoto-Fukuda et al. [58]). (a) One pgk-1 spot in the nuclei of epithelial cells was detected in male. (b) One or two pgk-1 spot(s) were detected in female cholesteatoma, respectively (reproduced with modification with permission from Yamamoto-Fukuda et al. [58]). (c) One or two pgk-1 spot(s) were detected in the epithelial nuclei of cholesteatoma (reproduced with modification with permission from Yamamoto-Fukuda et al. [58]). (d) On the other hand, one pgk-1 spot was detected in the cells of EAC (reproduced with modification with permission from Yamamoto-Fukuda et al. [58]). Magnification, ×350. Green arrows: two spots. Blue arrows: one spot.
