Pathogenicity of a Very Virulent Strain of Marek's Disease Herpesvirus Cloned as Infectious Bacterial Artificial Chromosomes
Figure 1
Southern hybridisation of wild-type and BAC clones of C12/130. Southern blots of the EcoRI-digested DNA from the wild-type C12/130 virus and the BAC clones sequentially hybridized with digoxigenin-labelled (a) gpt and (b) meq probe. While the gpt probe detects the specific band in all BAC-derived viral DNA, but not in the wild-type C12/130 virus, meq probe gives positive signals in all the lanes. Band sizes are shown on the right hand side. Lanes (1) pRB-1B5 [4], (2) pC12-130-3, (3) pC12-130-5, (4) pC12-130-8, (5) pC12-130-10, (6) pC12-130-15, (7) wild-type C12-130, (8) virus derived from pC12-130-10, and (9) pC12-130-15.
