Figure 1: Intravacuolar organization of tachyzoites in MDCK cells. (a) Phase contrast micrograph of a rosette of intravacuolar tachyzoites and (b) SEM-Tanaka micrographs of a rosette (B). Long arrows indicate the RB; arrowheads show intravacuolar network extensions connecting parasites to the PVM; short arrows indicate the position of the apical end in tachyzoites; asterisks show the empty space observed around parasites, at the periphery of the PV. The counterpart of the PV was devoid of any material from the MNN or of tachyzoites (c). μm.