Figure 1: Insert DNA analysis of random BAC clones from the Camellia sinensis HindIII BAC library, CSBCBa, by pulsed-field gel electrophoresis. DNA samples were digested with NotI and separated by pulsed-field gel electrophoresis with ramp pulse time of 5–15 s at 6 V/cm at 14°C in 0.5× TBE buffer for 16 h. Markers used are midrange I (outside lanes). The 7.5-kb common band corresponds to the cloning vector pIndigoBAC536 SwaI (pAGIBAC1).