Construction, Characterization, and Preliminary BAC-End Sequence Analysis of a Bacterial Artificial Chromosome Library of the Tea Plant (<i>Camellia sinensis</i>)

<table>Insert DNA analysis of random BAC clones from the <i>Camellia sinensis  Hind</i>III BAC library, CSBCBa, by pulsed-field gel electrophoresis. DNA samples were digested with <i>Not</i>I and separated by pulsed-field gel electrophoresis with ramp pulse time of 5–15 s at 6 V/cm at 14°C in 0.5× TBE buffer for 16 h. Markers used are midrange I (outside lanes). The 7.5-kb common band corresponds to the cloning vector pIndigoBAC536 <i>Swa</i>I (pAGIBAC1).</table>

BioMed Research International

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Figure 1

Figure 1: Construction, Characterization, and Preliminary BAC-End Sequence Analysis of a Bacterial Artificial Chromosome Library of the Tea Plant (<i>Camellia sinensis</i>) 

Figure 1 | Construction, Characterization, and Preliminary BAC-End Sequence Analysis of a Bacterial Artificial Chromosome Library of the Tea Plant (Camellia sinensis) 