Figure 2: Neural marker expression of hMSCs during neural differentiation. (a) Immunocytochemical analysis of differentiated hMSC with neuronal lineage-specific antibodies (×300 objective). Cells that had been harvested 72 hr after changing to a NDM were stained with the primary antibodies listed in Materials and methods. Hoechst 33328 was used for nuclear counterstaining. (b) Quantification of the percentage of stained cells from two different slides (10 fields of view, except in the case of O4 with 5 fields and Tuj-1 with 8 fields). Data represent mean ± SD. *   ** . NGM: normal growth media; NIM: neural induction media; NDM: neural differentiation media.