Figure 5: Cell count and cell cycle transition of hMSCs during neural differentiation. (a) Cell counts were determined 36 hr after changing to a NDM (corresponding to 76 hr after culturing in NGM and to 60 hr after changing to a NIM). Data represent means ± SD ( ). * . (b) The numbers of death cells were determined by counting the trypan blue stained cells in a hemocytometer. (c) Flow cytometric cell cycle analysis of hMSCs cultured under 3 different culture conditions. DNA content-related cell cycle distribution of hMSCs was determined 24 hr after changing to a NDM. Data represent means ± SD ( ). * . NGM: normal growth media; NIM: neural induction media; NDM: neural differentiation media.