Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells
Figure 1
SAA localizes with microtubules in untreated HCAEC, but not with actin filaments or vimentin. In HCAEC Von Willebrand factor (vWB) is demonstrated (red) in all cells (a). In (b) labeling of actin filaments, SAA, and nuclei is presented. In (c) (stack of optical sections) and (e) (larger magnification) SAA labeling can be detected attached to microtubules MT (arrowheads). Vimentin labeling together with SAA does not show any specific colocalization ((d)—stack of optical sections and (f) larger magnification). In (b, c, d, e and f) the labeling of SAA is red, of cytoskeletal elements is green, nuclei is blue. With PLA method colocalization of SAA with tubulin ((g)-TUB) or with vimentin ((h)-VIM) resulted in red reaction product strongly expressed only for tubulin and SAA. Anti-SAA polyclonal antibodies 3, 5, and 6, which target SAA peptides with amino acid sequences 27–44, 59–72, and 68–84, respectively, were used in the designated SAA panels. Bar = 10 μm.
