Figure 2: Quantification of cerebral amyloid accumulation in PS2Tg2576 mice. (a) Number of amyloid plaques (counts per cross section of half brain ± s.e.m) according to 82E1 antibody staining ( ). ELISA measurement of Aβ42 (b) and Aβ40 (c) in the TBS-insoluble fraction prepared from whole mouse brains ( ). The values are expressed as mean ± s.e.m ng per mg of protein. Statistical significance was assessed using the unpaired -test and Welch's test: * compared with the Tg2576 mice at 2-3 months of age. No other differences were found.