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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 631268, 8 pages
http://dx.doi.org/10.1155/2011/631268
Research Article

MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors

1Genetics Division, Department of Morphology and Genetics, Federal University of São Paulo, 04023-900 São Paulo, SP, Brazil
2Human Cytogenetics Laboratory, Institute of Biological Sciences, Federal University of Pará, 66073-000 Belém, PA, Brazil
3Surgery Service, João de Barros Barreto University Hospital, Federal University of Pará, 60673-000 Belém, PA, Brazil

Received 11 September 2010; Revised 23 December 2010; Accepted 8 January 2011

Academic Editor: Yataro Daigo

Copyright © 2011 Mariana Ferreira Leal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We evaluated whether MYC, TP53, and chromosome 17 copy-number alterations occur in ACP02, ACP03, and AGP01 gastric cancer cell lines and in their tumor counterpart. Fluorescence in situ hybridization for MYC and TP53 genes and for chromosome 17 was applied in the 6th, 12th, 60th, and 85th passages of the cell lines and in their parental primary tumors. We observed that three and four MYC signals were the most common alterations in gastric cell lines and tumors. ACP02 presented cells with two copies of chr17 and loss of one copy of TP53 more frequently than ACP03 and AGP01. Only ACP03 and AGP01 presented clonal chr17 trisomy with three or two TP53 copies. The frequency of MYC gain, TP53 loss, and chromosome 17 trisomy seems to increase in gastric cell lines compared to their parental tumors. Our findings reveal that these cell lines retain, in vitro, the genetic alterations presented in their parental primary tumors.