Research Article

Transcription Factor Sp1 Is Involved in Expressional Regulation of Coxsackie and Adenovirus Receptor in Cancer Cells

Figure 4

Effects of MTM on CAR promoter activity and mRNA expressions. HCT116 (a), HeLa (c), and HepG2 (e) were treated with either MTM or DMSO (vehicle) for 24 h at the concentration indicated. The mRNA expressions of CAR and GAPDH used as an internal control were examined by Semiquantitative RT-PCR. Core promoter reporter construct was cotransfected with β-galactosidase into the three different cells and followed by treatment with either MTM or DMSO for 24 h at the concentration indicated (b, d, and f). The relative luciferase activity was determined by statistical analysis of three independent experiments. Bars represent the mean ± SD.
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