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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 692846, 8 pages
http://dx.doi.org/10.1155/2011/692846
Research Article

Synergic Effect of Genistein and Daidzein on UVB-Induced DNA Damage: An Effective Photoprotective Combination

1Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli “Federico II”, Via S. Pansini 5, 80131 Napoli, Italy
2CEINGE—Biotecnologie Avanzate, Via Comunale Margherita 482, 80145 Napoli, Italy
3Rottapharm-Madaus Dermo-Cosmetic R&D Division, Via Valosa di Sopra 9, 20052 Monza, Italy
4Dipartimento di Patologia Sistematica, Sezione di Dermatologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli “Federico II”, Via S. Pansini 5, 80131 Napoli, Italy
5Facoltà di Scienze Biotecnologiche, Università degli Studi di Napoli “Federico II”, Via S. Pansini 5, 80131 Napoli, Italy

Received 10 January 2011; Revised 11 March 2011; Accepted 19 May 2011

Academic Editor: Susan A. Rotenberg

Copyright © 2011 Barbara Iovine et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The anti-inflammatory effects and antioxidant activities of individual isoflavones are well established although little is known about the photoprotective effect of their combination. The aim of this study was to investigate the photoprotective effects of different concentrations of genistein and daidzein individually or combined. We measured the expression levels of the cyclo-oxygenase-2 (COX-2) and growth arrest and DNA-damage inducible (Gadd45) genes, which are involved in inflammation and DNA repair, respectively, in BJ-5ta human skin fibroblasts irradiated with 60 mJ/cm2 UVB. We also determined the cellular response to UVB-induced DNA damage by Comet assay. We report that genistein and daidzein when administered combined, and at a specific concentration and ratio, exerted a synergistic photoprotective effect that was greater than the effect obtained with each isoflavone alone. The results reported herein suggest that low concentrations of genistein and daidzein combined may be good candidate ingredients for protective agents against UV-induced photodamage.