Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations

Table 4

Optimum conditions predicted by ANNs in SmF and SSF systems.

	T (°C)	pH	IS (%)	t (h)	Agt (rpm)	Predicted Lipase activity (U/mg protein)	Actual Lipase activity (U/mg protein)
SmF	29	6.0	1	24	200	31.0	32.2
	T (°C)	pH	M (%)	t (h)	Oo (%)	Predicted Lipase activity (U/mg protein)	Actual Lipase activity (U/mg protein)
SSF	30	6.5	90	81.6	5	79.0	75.4

AAD for SmF: 0.04%; AAD for SSF: 0.05%.
T: growth temperature (°C), pH: initial pH, IS: inoculum size (%), t: incubation period (h), Agt: agitation rate (rpm), Oo: olive oil (%), M: moisture content (%).