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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 706746, 10 pages
Research Article

Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy

1Dipartimento di Medicina Clinica, Scienze Cardiovascolari e Immunologiche e Centro Interdipartimentale di Ricerca in Immunologia di Base e Clinica (CISI), Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy
2Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, 80131 Napoli, Italy
3Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Napoli, Italy
4CEINGE Biotecnologie Avanzate, Via G. Salvatore 486, 80145 Napoli, Italy

Received 19 May 2011; Accepted 5 July 2011

Academic Editor: Leonid Medved

Copyright © 2011 Giuseppe Spadaro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.